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	Provedor de dados BJM (2) Autor Bon,Elba Pinto da Silva (2) Carvajal,Elvira (1) Gottschalk,Leda Maria Fortes (1) Paredes,Raquel de Sousa (1) Silva,Ayla Sant'Ana da (1) Sotero-Martins,Adriana (1) Teixeira,Ricardo Sposina Sobral (1) Mais... Palavra-chave Asparaginase II (1) Aspergillus awamori 2B.361 U2/1 (1) Cellulases (1) Genomic integration (1) Green fluorescent protein (1) Hemicellulases (1) Nitrogen nutrition (1) Nitrogen regulation (1) Saccharomyces cerevisiae (1) Β-glucosidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation BJM Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira. Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent protein. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017 Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1 BJM Gottschalk,Leda Maria Fortes; Paredes,Raquel de Sousa; Teixeira,Ricardo Sposina Sobral; Silva,Ayla Sant'Ana da; Bon,Elba Pinto da Silva. The production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, β-xylosidase and ferulic acid esterase to a peak concentrations of 44,880; 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus awamori 2B.361 U2/1; Cellulases; Hemicellulases; Β-glucosidase; Nitrogen nutrition. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200037 Registros recuperados: 2 Primeira ... 1 ... Última

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